| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-tropomyosin 4/TPM4 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 32kDa band observed in Human Platelets, Pig Heart and in NIH3T3 lysates (calculated MW of 32.7kDa according to NP_001138632.1). Recommended concentration: 0.1-0.3µg/ml. Primary |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TPM4 |
| Gene ID: | 7171 |
| Uniprot ID: | TPM4_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_001138632.1; NP_003281.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1). |
| Immunogen Sequence: | ENVGLHQTLDQ |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. Plays a role in platelet biogenesis. |
| Protein Name | Tropomyosin Alpha-4 ChainTm30p1Tropomyosin-4 |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-445355Reactome: R-HSA-9013424Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmCytoskeletonAssociates With F-Actin Stress Fibers |
| Alternative Antibody Names | Anti-Tropomyosin Alpha-4 Chain antibodyAnti-Tm30p1 antibodyAnti-Tropomyosin-4 antibodyAnti-TPM4 antibody |
Information sourced from Uniprot.org
