• Western blot analysis of extracts of various cell lines, using TROP-2 antibody (STJ11103619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
  • Immunofluorescence analysis of A431 cells using TROP-2 Rabbit polyclonal antibody (STJ11103619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse kidney, using TROP-2 antibody (STJ11103619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using TROP-2 Rabbit polyclonal antibody (STJ11103619) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using TROP-2 rabbit polyclonal antibody (STJ11103619) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse kidney, using TROP-2 antibody (STJ11103619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of A431 cells using TROP-2 rabbit polyclonal antibody (STJ11103619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TROP-2 antibody (STJ11103619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-TROP-2 antibody (27-270) (STJ11103619)

SKU:
STJ11103619

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TROP-2 (27-270) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TACSTD2
Gene ID: 4070
Uniprot ID: TACD2_HUMAN
Immunogen Region: 27-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-270 of human TROP-2 (NP_002344.2).
Immunogen Sequence: HTAAQDNCTCPTNKMTVCSP DGPGGRCQCRALGSGMAVDC STLTSKCLLLKARMSAPKNA RTLVRPSEHALVDNDGLYDP DCDPEGRFKARQCNQTSVCW CVNSVGVRRTDKGDLSLRCD ELVRTHHILIDLRHRPTAGA FNHSDLDAELRRLFRERYRL HPKFVAAVHYEQPTIQIELR QNTSQKAAGDVDIGDAAYYF ERDIKGESLFQGRGGLDLRV RGEPLQVERTLIYYLDEIP
Tissue Specificity Placenta, pancreatic carcinoma cell lines.
Post Translational Modifications The N-terminus is blocked.
Function May function as a growth factor receptor.
Protein Name Tumor-Associated Calcium Signal Transducer 2
Cell Surface Glycoprotein Trop-2
Membrane Component Chromosome 1 Surface Marker 1
Pancreatic Carcinoma Marker Protein Ga733-1
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tumor-Associated Calcium Signal Transducer 2 antibody
Anti-Cell Surface Glycoprotein Trop-2 antibody
Anti-Membrane Component Chromosome 1 Surface Marker 1 antibody
Anti-Pancreatic Carcinoma Marker Protein Ga733-1 antibody
Anti-TACSTD2 antibody
Anti-GA733-1 antibody
Anti-M1S1 antibody
Anti-TROP2 antibody

Information sourced from Uniprot.org

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