• Biotinylated STJ72398 (0. 01ug/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-TRIM72, Biotinylated antibody (Internal) {Biotin} (STJ73502)

SKU:
STJ73502-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TRIM72, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM72
Gene ID: 493829
Uniprot ID: TRI72_HUMAN
Immunogen Region: Internal
Accession Number: NP_001008275.1
Immunogen Sequence: RPQALSTNLQ.
Function Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
Protein Name Tripartite Motif-Containing Protein 72
Mitsugumin-53
Mg53
Database Links Reactome: R-HSA-445355
Cellular Localisation Cell Membrane
Sarcolemma
Cytoplasmic Vesicle Membrane
Tethered To Plasma Membrane And Cytoplasmic Vesicles Via Its Interaction With Phosphatidylserine
Alternative Antibody Names Anti-Tripartite Motif-Containing Protein 72 antibody
Anti-Mitsugumin-53 antibody
Anti-Mg53 antibody
Anti-TRIM72 antibody
Anti-MG53 antibody

Information sourced from Uniprot.org

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