Anti-TRIM65 antibody (380-490 aa) (STJ11106123)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRIM65 (380-490 aa) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM65 |
| Gene ID | 201292 |
| Uniprot ID | TRI65_HUMAN |
| Immunogen Region | 380-490 aa |
| Immunogen Sequence | QAGHHYWEVRASDHSVTLGV SYPQLPRCRLGPHTDNIGRG PCSWGLCVQEDSLQAWHNGE AQRLPGVSGRLLGMDLDLAS GCLTFYSLEPQTQPLYTFHA LFNQPLTPVFW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 380-490 of human TRIM65 (NP_775818.2). |
Additional Info
| Function | E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs. Plays also a role in limitation of inflammation through different mechanisms. First, promotes 'Lys-48'-mediated ubiquitination of VCAM1 leading to its degradation and limitation of LPS-induced lung inflammation. In addition, negatively regulates inflammasome activation by promoting 'lys48'-linked ubiquitination of NLRP3 which is critical for the inhibition of NLRP3 inflammasome activation in resting macrophages. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim65Tripartite Motif-Containing Protein 65 |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim65 antibodyAnti-Tripartite Motif-Containing Protein 65 antibodyAnti-TRIM65 antibody |
Information sourced from Uniprot.org