| Host: | Mouse |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-MuRF1 (C-Term) is suitable for use in Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M316 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:250 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM63 |
| Gene ID: | 84676 |
| Uniprot ID: | TRI63_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | The antibody detects a full length human MuRF1 recombinant protein on SDS-PAGE immunoblots. |
| Immunogen: | Clone M316 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55). |
| Tissue Specificity | Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. |
| Function | E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim63 Iris Ring Finger Protein Muscle-Specific Ring Finger Protein 1 Murf-1 Murf1 Ring Finger Protein 28 Ring-Type E3 Ubiquitin Transferase Trim63 Striated Muscle Ring Zinc Finger Protein Tripartite Motif-Containing Protein 63 |
| Database Links | Reactome: R-HSA-9615017 Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Nucleus Myofibril Sarcomere M Line Z Line Colocalizes With Tnni3 In Myocytes Localizes To The M- And Z-Lines In Skeletal Muscle |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim63 antibody Anti-Iris Ring Finger Protein antibody Anti-Muscle-Specific Ring Finger Protein 1 antibody Anti-Murf-1 antibody Anti-Murf1 antibody Anti-Ring Finger Protein 28 antibody Anti-Ring-Type E3 Ubiquitin Transferase Trim63 antibody Anti-Striated Muscle Ring Zinc Finger Protein antibody Anti-Tripartite Motif-Containing Protein 63 antibody Anti-TRIM63 antibody Anti-IRF antibody Anti-MURF1 antibody Anti-RNF28 antibody Anti-SMRZ antibody |
Information sourced from Uniprot.org