| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRIM22 (215-411) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRIM22 |
| Gene ID: |
10346 |
| Uniprot ID: |
TRI22_HUMAN |
| Immunogen Region: |
215-411 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 215-411 of human TRIM22 (NP_006065.2). |
| Immunogen Sequence: |
NLAAATDQLVQQRQDASTLI SDLQRRLRGSSVEMLQDVID VMKRSESWTLKKPKSVSKKL KSVFRVPDLSGMLQVLKELT DVQYYWVDVMLNPGSATSNV AISVDQRQVKTVRTCTFKNS NPCDFSAFGVFGCQYFSSGK YYWEVDVSGKIAWILGVHSK ISSLNKRKSSGFAFDPSVNY SKVYSRYRPQYGYWVIG |
| Tissue Specificity | Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues. |
| Post Translational Modifications | Auto-ubiquitinated. |
| Function | Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity. Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A. Acts also by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation. Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter. Plays also a role in antiviral immunity by co-regulating together with NT5C2 the RIGI/NF-kappa-B pathway by promoting 'Lys-63'-linked ubiquitination of RIGI, while NT5C2 is responsible for 'Lys-48'-linked ubiquitination of RIGI. Participates in adaptive immunity by suppressing the amount of MHC class II protein in a negative feedback manner in order to limit the extent of MHC class II induction. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim2250 Kda-Stimulated Trans-Acting FactorRing Finger Protein 94Ring-Type E3 Ubiquitin Transferase Trim22Staf-50Tripartite Motif-Containing Protein 22 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleCajal BodyLocalizes Predominantly To The NucleusFound In Cytoplasm To Some ExtentForms Distinct Nuclear Bodies That Undergo Dynamic Changes During Cell Cycle ProgressionNuclear Bodies Start To Form In The Early G0/G1 Phase But Become Speckle-Like In The S-Phase And Completely Dispersed In Mitosis35% Of Trim22 Nuclear Bodies Overlap Or Are Found Adjacent To Cajal Bodies |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim22 antibodyAnti-50 Kda-Stimulated Trans-Acting Factor antibodyAnti-Ring Finger Protein 94 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim22 antibodyAnti-Staf-50 antibodyAnti-Tripartite Motif-Containing Protein 22 antibodyAnti-TRIM22 antibodyAnti-RNF94 antibodyAnti-STAF50 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
