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1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200
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Immunofluorescence analysis of Rat-liver tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Western blot analysis of Hela, diluted at 1) 1:2000 , 2) 1:5000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
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Immunofluorescence analysis of Human-appendix tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, Bek Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
