• Immunohistochemistry analysis of TRAPPC6B in paraffin-embedded Mouse brain using TRAPPC6B Rabbit polyclonal antibody (STJ117756) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TRAPPC6B in paraffin-embedded Rat brain using TRAPPC6B Rabbit polyclonal antibody (STJ117756) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TRAPPC6B Rabbit polyclonal antibody (STJ117756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRAPPC6B antibody (1-130) (STJ117756)

SKU:
STJ117756

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRAPPC6B (1-130) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRAPPC6B
Gene ID: 122553
Uniprot ID: TPC6B_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TRAPPC6B (NP_803235.1).
Immunogen Sequence: MADEALFLLLHNEMVSGVYK SAEQGEVENGRCITKLENMG FRVGQGLIERFTKDTARFKD ELDIMKFICKDFWTTVFKKQ IDNLRTNHQYLAFTCGLIRG GLSNLGIKSIVTAEVSSMPA CKFQVMIQKL
Tissue Specificity Both isoforms are expressed ubiquitously (at transcript level), isoform 1 being the most predominant. Expressed in the fetal brain and different regions of the adult brain and spinal cord.
Function Component of a transport protein particle (TRAPP) complex that may function in specific stages of inter-organelle traffic. Specifically involved in the early development of neural circuitry, likely by controlling the frequency and amplitude of intracellular calcium transients implicated in the regulation of neuron differentiation and survival (Probable).
Protein Name Trafficking Protein Particle Complex Subunit 6b
Trapp Complex Subunit 6b
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-8876198
Cellular Localisation Golgi Apparatus
Cis-Golgi Network
Endoplasmic Reticulum
Alternative Antibody Names Anti-Trafficking Protein Particle Complex Subunit 6b antibody
Anti-Trapp Complex Subunit 6b antibody
Anti-TRAPPC6B antibody

Information sourced from Uniprot.org

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