Anti-TRAK1 antibody (N-Term) (STJ13102523)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from n-terminal region of mouse TRAK1 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-TRAK1 (N-Term) for use in IHC and WB in Mouse, Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Trak1 |
| Gene ID | 67095 |
| Uniprot ID | TRAK1_MOUSE |
| Immunogen | A synthetic peptide from n-terminal region of mouse TRAK1 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | N-Term |
| Specificity | Specific for TRAK1. |
Additional Info
| Post Translational Modifications | O-glycosylated. Glycosylated by OGT.glycosylation in response to increased extracellular glucose levels is required for and leads to regulation of mitochondrial motility by OGT. |
| Function | Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors. Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes. TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria. |
| Protein Name | Trafficking Kinesin-Binding Protein 1Protein Milton |
| Database Links | Reactome: R-MMU-9013419Reactome: -MMU-9013425 |
| Cellular Localisation | CytoplasmNucleusMitochondrionEarly EndosomeEndosomeMitochondrion MembraneCell CortexPredominantly Associated With Early EndosomeThe Localization To Early Endosomes Depends On Its Interaction With Hgs/HrsColocalizes With Mgarp At The Mitochondria |
| Alternative Antibody Names | Anti-Trafficking Kinesin-Binding Protein 1 antibodyAnti-Protein Milton antibodyAnti-Trak1 antibody |
Information sourced from Uniprot.org