Anti-TPO-R/CD110/c-Mpl antibody (536-635) [S6MR] (STJ11103096)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal TPO-R/CD110/c-Mpl (536-635) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S6MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MPL |
| Gene ID | 4352 |
| Uniprot ID | TPOR_HUMAN |
| Immunogen Region | 536-635 |
| Immunogen Sequence | HRVLGQYLRDTAALSPPKAT VSDTCEEVEPSLLEILPKSS ERTPLPLCSSQAQMDYRRLQ PSCLGTMPLSVCPPMAESGS CCTTHIANHSYLPLSYWQQP |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 536-635 of human TPO-R/CD110/c-Mpl (P40238). |
Additional Info
| Tissue Specificity | Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed. |
| Post Translational Modifications | Phosphorylated at Tyr-591 in response to THPO stimulation. Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. |
| Function | Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K. In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production. |
| Protein Name | Thrombopoietin ReceptorTpo-RMyeloproliferative Leukemia ProteinProto-Oncogene C-MplCd Antigen Cd110 |
| Database Links | Reactome: R-HSA-76009 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusCell Surface |
| Alternative Antibody Names | Anti-Thrombopoietin Receptor antibodyAnti-Tpo-R antibodyAnti-Myeloproliferative Leukemia Protein antibodyAnti-Proto-Oncogene C-Mpl antibodyAnti-Cd Antigen Cd110 antibodyAnti-MPL antibodyAnti-TPOR antibody |
Information sourced from Uniprot.org