| Host: |
Rabbit |
| Applications: |
ELISA/WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TPCN2 (680-731) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
FITC |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.55-0.57 µg/µl |
| Dilution Range: |
WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200IHC: 1:50-1:150 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Tpcn2 |
| Gene ID: |
233979 |
| Uniprot ID: |
TPC2_MOUSE |
| Immunogen Region: |
680-731 |
| Immunogen: |
Synthetic peptide taken within amino acid region 680-731 between TMD4 and TMD5 on mouse TPC2 protein. |
| Tissue Specificity | Widely expressed. Highly expressed in macrophages. Expressed in pigmented cells. |
| Post Translational Modifications | N-glycosylated. |
| Function | Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate). Localizes to the lysosomal and late endosome membranes where it regulates organellar membrane excitability, membrane trafficking, and pH homeostasis. Is associated with a plethora of physiological processes, including mTOR-dependent nutrient sensing, skin pigmentation and autophagy. Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2. As calcium channel, it increases the pH in the lysosomal lumen, as sodium channel, it promotes lysosomal exocytosis. Plays a crucial role in endolysosomal trafficking in the endolysosomal degradation pathway and is potentially involved in the homeostatic control of many macromolecules and cell metabolites. Also expressed in melanosomes of pigmented cells where mediates a Ca(2+) channel and/or PI(3,5)P2-activated melanosomal Na(+) channel to acidify pH and inhibit tyrosinase activity required for melanogenesis and pigmentation. Unlike the voltage-dependent TPCN1, TPCN2 is voltage independent and can be activated solely by PI(3,5)P2 binding. In contrast, PI(4,5)P2, PI(3,4)P2, PI(3)P and PI(5)P have no obvious effect on channel activation. (Microbial infection) During Ebola virus (EBOV) infection, controls the movement of endosomes containing virus particles and is required by EBOV to escape from the endosomal network into the cell cytoplasm. |
| Protein Name | Two Pore Channel Protein 2Two Pore Calcium Channel Protein 2Voltage-Dependent Calcium Channel Protein Tpc2 |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome MembraneMelanosome MembraneOnly The Acidic Lysosomal Fraction Is Sensitive To Naadp |
| Alternative Antibody Names | Anti-Two Pore Channel Protein 2 antibodyAnti-Two Pore Calcium Channel Protein 2 antibodyAnti-Voltage-Dependent Calcium Channel Protein Tpc2 antibodyAnti-Tpcn2 antibodyAnti-Tpc2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
