• Immunohistochemistry analysis of paraffin-embedded human brain using 53BP1 Rabbit polyclonal antibody (STJ11104450) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using TP53BP1 Rabbit polyclonal antibody (STJ11104450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-TP53BP1 antibody (895-1031) (STJ11104450)

SKU:
STJ11104450

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tp53-Binding Protein 1 (895-1031) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TP53BP1
Gene ID: 7158
Uniprot ID: TP53B_HUMAN
Immunogen Region: 895-1031
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 895-1031 of human TP53BP1 (NP_005648.1).
Immunogen Sequence: FCESSSETPFHFTLPKEGDI IPPLTGATPPLIGHLKLEPK RHSTPIGISNYPESTIATSD VMSESMVETHDPILGSGKGD SGAAPDVDDKLCLRMKLVSP ETEASEESLQFNLEKPATGE RKNGSTAVAESVASPQK
Post Translational Modifications Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Phosphorylated at basal level in the absence of DNA damage. Phosphorylated by ATM in response to DNA damage: phosphorylation at different sites promotes interaction with different set of proteins: phosphorylation at the N-terminus by ATM (residues from 6-178) promotes interaction with PAXIP1 and non-homologous end joining (NHEJ) of dysfunctional telomeres. Phosphorylation by ATM at residues that are located more C-terminus (residues 300-650) leads to promote interaction with RIF1. Interaction with RIF1 leads to disrupt interaction with NUDT16L1/TIRR. Phosphorylation at Thr-1609 and Ser-1618 in the UDR motif blocks interaction with H2AK15ub. Dephosphorylated by PPP4C. Hyperphosphorylation during mitosis correlates with its exclusion from chromatin and DNA lesions. Hyperphosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Dephosphorylated by PPP5C.
Function Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Protein Name Tp53-Binding Protein 1
53bp1
P53-Binding Protein 1
P53bp1
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To The Nucleus In Absence Of Dna Damage
Following Dna Damage
Recruited To Sites Of Dna Damage
Such As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)
Two Histone Marks That Are Present At Dsbs Sites
Associated With Kinetochores During Mitosis
Alternative Antibody Names Anti-Tp53-Binding Protein 1 antibody
Anti-53bp1 antibody
Anti-P53-Binding Protein 1 antibody
Anti-P53bp1 antibody
Anti-TP53BP1 antibody

Information sourced from Uniprot.org

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