• Western blot analysis of mouse heart, using Cardiac Troponin I (TNNI3) antibody (STJ114870) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of Rat lung, using Cardiac Troponin I (TNNI3) antibody (STJ114870) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of mouse heart cells using Cardiac Troponin I (TNNI3) antibody (STJ114870) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse heart cells using 3 Mu g Cardiac Troponin I (TNNI3) antibody (STJ114870). Western blot was performed from the immunoprecipitate using Cardiac Troponin I (TNNI3) antibody (STJ114870) at a dilution of 1:1000.

Anti-TNNI3 antibody (1-210) (STJ114870)

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STJ114870

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNNI3 (1-210) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNNI3
Gene ID: 7137
Uniprot ID: TNNI3_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human Cardiac Troponin I (TNNI3) (NP_000354.4).
Immunogen Sequence: MADGSSDAAREPRPAPAPIR RRSSNYRAYATEPHAKKKSK ISASRKLQLKTLLLQIAKQE LEREAEERRGEKGRALSTRC QPLELAGLGFAELQDLCRQL HARVDKVDEERYDIEAKVTK NITEIADLTQKIFDLRGKFK RPTLRRVRISADAMMQALLG ARAKESLDLRAHLKQVKKED TEKENREVGDWRKNIDALSG MEGRKKKFES
Post Translational Modifications Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name Troponin I - Cardiac Muscle
Cardiac Troponin I
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-5578775
Alternative Antibody Names Anti-Troponin I - Cardiac Muscle antibody
Anti-Cardiac Troponin I antibody
Anti-TNNI3 antibody
Anti-TNNC1 antibody

Information sourced from Uniprot.org

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