| Short Description : | Rabbit monoclonal Troponin I-Cardiac Muscle (1-210) antibody for use in IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S4241RM |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TNNI3 |
| Gene ID: | 7137 |
| Uniprot ID: | TNNI3_HUMAN |
| Immunogen Region: | 1-210 |
| Immunogen Sequence: | MADGSSDAAREPRPAPAPIR RRSSNYRAYATEPHAKKKSK ISASRKLQLKTLLLQIAKQE LEREAEERRGEKGRALSTRC QPLELAGLGFAELQDLCRQL HARVDKVDEERYDIEAKVTK NITEIADLTQKIFDLRGKFK RPTLRRVRISADAMMQALLG ARAKESLDLRAHLKQVKKED TEKENREVGDWRKNIDALSG MEGRKKKFES |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human Cardiac Troponin I (TNNI3) (NP_000354.4). |
| Post Translational Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-5578775 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Troponin I - Cardiac Muscle antibodyAnti-Cardiac Troponin I antibodyAnti-TNNI3 antibodyAnti-TNNC1 antibody |
Information sourced from Uniprot.org
