Western blot analysis of extracts of various cell lines, using CD30 antibody (STJ29965) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TNFRSF8 antibody (500-595) (STJ29965)

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STJ29965
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFRSF8
Gene ID: 943
Uniprot ID: TNR8_HUMAN
Immunogen Region: 500-595
Specificity: A synthetic peptide corresponding to a sequence within amino acids 500-595 of human CD30 (NP_001234.3).
Immunogen Sequence: LPEPRVSTEHTNNKIEKIYI MKADTVIVGTVKAELPEGRG LAGPAEPELEEELEADHTPH YPEQETEPPLGSCSDVMLSV EEEGKEDPLPTAASGK
Tissue Specificity Isoform 2: Detected in alveolar macrophages (at protein level).
Post Translational Modifications Phosphorylated on serine and tyrosine residues (Probable). Isoform 2 is constitutively phosphorylated.
Function Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 8
Cd30l Receptor
Ki-1 Antigen
Lymphocyte Activation Antigen Cd30
Cd Antigen Cd30
Database Links Reactome: R-HSA-5669034
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 8 antibody
Anti-Cd30l Receptor antibody
Anti-Ki-1 Antigen antibody
Anti-Lymphocyte Activation Antigen Cd30 antibody
Anti-Cd Antigen Cd30 antibody
Anti-TNFRSF8 antibody
Anti-CD30 antibody
Anti-D1S166E antibody

Information sourced from Uniprot.org

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