Anti-TLR6 antibody (195-225) (STJ503280)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 195-225 on human TLR6 protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-TLR6 (195-225) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Cat/Human/Mouse/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.78-0.80 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:20, 000ELISA: 1:20, 000IP: 1:200IHC: 1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TLR6 |
| Gene ID | 10333 |
| Uniprot ID | TLR6_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 195-225 on human TLR6 protein. |
| Immunogen Region | 195-225 |
Additional Info
| Tissue Specificity | Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells. |
| Function | Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides. In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. |
| Protein Name | Toll-Like Receptor 6Cd Antigen Cd286 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-168188Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmic VesiclePhagosome MembraneMembrane RaftGolgi ApparatusUpon Complex Formation With Cd36 And Tlr4Internalized Through Dynamin-Dependent EndocytosisDoes Not Reside In Lipid Rafts Before Stimulation But Accumulates Increasingly In The Raft Upon The Presence Of The Microbial LigandIn Response To Diacylated LipoproteinsTlr2:Tlr6 Heterodimers Are Recruited In Lipid RaftsThis Recruitment Determine The Intracellular Targeting To The Golgi Apparatus |
| Alternative Antibody Names | Anti-Toll-Like Receptor 6 antibodyAnti-Cd Antigen Cd286 antibodyAnti-TLR6 antibody |
Information sourced from Uniprot.org