Anti-TLR5 antibody (714-858) (STJ119345)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TLR5 (714-858) antibody for use in WB, IHC-P and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLR5 |
| Gene ID | 7100 |
| Uniprot ID | TLR5_HUMAN |
| Immunogen Region | 714-858 |
| Immunogen Sequence | HLDTQYSDQNRFNLCFEERD FVPGENRIANIQDAIWNSRK IVCLVSRHFLRDGWCLEAFS YAQGRCLSDLNSALIMVVVG SLSQYQLMKHQSIRGFVQKQ QYLRWPEDLQDVGWFLHKLS QQILKKEKEKKKDNNIPLQT VATIS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 714-858 of human TLR5 (NP_003259.2). |
Additional Info
| Tissue Specificity | Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells. |
| Post Translational Modifications | Phosphorylated at Ser-805 by PKD/PRKD1.phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding.required for signaling. |
| Function | Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life. |
| Protein Name | Toll-Like Receptor 5Toll/Interleukin-1 Receptor-Like Protein 3 |
| Database Links | Reactome: R-HSA-168176Reactome: R-HSA-5602680Reactome: R-HSA-5603037Reactome: R-HSA-975871 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Toll-Like Receptor 5 antibodyAnti-Toll/Interleukin-1 Receptor-Like Protein 3 antibodyAnti-TLR5 antibodyAnti-TIL3 antibody |
Information sourced from Uniprot.org