• Immunofluorescence analysis of U2OS cells using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using TLR1 Rabbit polyclonal antibody (STJ25862) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TLR1 antibody (STJ25862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TLR1 antibody (30-290) (STJ25862)

SKU:
STJ25862

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TLR1 (30-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR1
Gene ID: 7096
Uniprot ID: TLR1_HUMAN
Immunogen Region: 30-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-290 of human TLR1 (NP_003254.2).
Immunogen Sequence: DRSKNGLIHVPKDLSQKTTI LNISQNYISELWTSDILSLS KLRILIISHNRIQYLDISVF KFNQELEYLDLSHNKLVKIS CHPTVNLKHLDLSFNAFDAL PICKEFGNMSQLKFLGLSTT HLEKSSVLPIAHLNISKVLL VLGETYGEKEDPEGLQDFNT ESLHIVFPTNKEFHFILDVS VKTVANLELSNIKCVLEDNK CSYFLSILAKLQTNPKLSNL TLNNIETTWNSFIRILQLV
Tissue Specificity Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
Function Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Protein Name Toll-Like Receptor 1
Toll/Interleukin-1 Receptor-Like Protein
Til
Cd Antigen Cd281
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1461957
Reactome: R-HSA-166058
Reactome: R-HSA-168179
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-9705671
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Phagosome Membrane
Membrane Raft
Golgi Apparatus
Does Not Reside In Lipid Rafts Before Stimulation But Accumulates Increasingly In The Raft Upon The Presence Of The Microbial Ligand
In Response To Triacylated Lipoproteins
Tlr2:Tlr1 Heterodimers Are Recruited In Lipid Rafts
This Recruitment Determine The Intracellular Targeting To The Golgi Apparatus
Alternative Antibody Names Anti-Toll-Like Receptor 1 antibody
Anti-Toll/Interleukin-1 Receptor-Like Protein antibody
Anti-Til antibody
Anti-Cd Antigen Cd281 antibody
Anti-TLR1 antibody
Anti-KIAA0012 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance