Western blot analysis of various lysates, using TLE4 Rabbit monoclonal antibody (STJ11105208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TLE4 antibody (1-138) [S5208RM] (STJ11105208)

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STJ11105208
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5208RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLE4
Gene ID: 7091
Uniprot ID: TLE4_HUMAN
Immunogen Region: 1-138
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human TLE4 (NP_001269677).
Immunogen Sequence: MIRDLSKMYPQTRHPAPHQP AQPFKFTISESCDRIKEEFQ FLQAQYHSLKLECEKLASEK TEMQRHYVMYYEMSYGLNIE MHKQAEIVKRLNAICAQVIP FLSQEHQQQVVQAVERAKQV TMAELNAIIGQQLQAQHL
Tissue Specificity In all tissues examined, mostly in brain, and muscle.
Post Translational Modifications Phosphorylated. PAX5 binding increases phosphorylation. Ubiquitinated by XIAP/BIRC4.
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression. Involved in transcriptional repression of GNRHR and enhances MSX1-mediated transcriptional repression of CGA/alpha-GSU.
Protein Name Transducin-Like Enhancer Protein 4
Grg-4
Groucho-Related Protein 4
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-2122947
Reactome: R-HSA-3769402
Reactome: R-HSA-4641265
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transducin-Like Enhancer Protein 4 antibody
Anti-Grg-4 antibody
Anti-Groucho-Related Protein 4 antibody
Anti-TLE4 antibody
Anti-GRG4 antibody
Anti-KIAA1261 antibody

Information sourced from Uniprot.org

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