Western blot analysis of lysates from U-87MG cells, using TIMM8A Rabbit pAb (STJ111853) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-TIMM8A antibody (1-97) (STJ111853)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111853
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General Information

Short DescriptionRabbit polyclonal TIMM8A (1-97) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTIMM8A
Gene ID1678
Uniprot IDTIM8A_HUMAN
Immunogen Region1-97
Immunogen SequenceMDSSSSSSAAGLGAVDPQLQ HFIEVETQKQRFQQLVHQMT ELCWEKCMDKPGPKLDSRAE ACFVNCVERFIDTSQFILNR LEQTQKSKPVFSESLSD
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human TIMM8A (NP_004076.1).

Additional Info

Tissue Specificity Highly expressed in fetal and adult brain, followed by fetal lung, liver and kidney. Also expressed in heart, placenta, lung, liver, kidney, pancreas, skeletal muscle and heart.
Function Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim8 A
Deafness Dystonia Protein 1
X-Linked Deafness Dystonia Protein
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Intermembrane Side
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim8 A antibody
Anti-Deafness Dystonia Protein 1 antibody
Anti-X-Linked Deafness Dystonia Protein antibody
Anti-TIMM8A antibody
Anti-DDP antibody
Anti-DDP1 antibody
Anti-TIM8A antibody

Information sourced from Uniprot.org

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