| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Themis-Mouse (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 72kDa band observed in Mouse Spleen lysates (calculated MW of 72.7kDa according to NP_848781.1). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: | C-Term |
| Accession Number: | NP_848781.1 |
| Immunogen Sequence: | EERTINLPKSLKSH |
| Post Translational Modifications | Phosphorylated on Tyr residues quickly after TCR stimulation. |
| Function | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. |
| Protein Name | Protein ThemisGrb2-Associating ProteinGaspProtein ThylexThymocyte-Expressed Molecule Involved In Selection |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Protein Themis antibodyAnti-Grb2-Associating Protein antibodyAnti-Gasp antibodyAnti-Protein Thylex antibodyAnti-Thymocyte-Expressed Molecule Involved In Selection antibodyAnti-Themis antibody |
Information sourced from Uniprot.org