• Immunofluorescence analysis of A-431 cells using CD141/Thrombomodulin Rabbit polyclonal antibody (STJ25842) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using CD141/Thrombomodulin Rabbit polyclonal antibody (STJ25842) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using CD141/Thrombomodulin Rabbit polyclonal antibody (STJ25842) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using CD141/Thrombomodulin Rabbit polyclonal antibody (STJ25842) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD141/Thrombomodulin antibody (STJ25842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of A-431 cells, using CD141/Thrombomodulin antibody (STJ25842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-THBD antibody (19-575) (STJ25842)

SKU:
STJ25842

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-THBD (19-575) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: THBD
Gene ID: 7056
Uniprot ID: TRBM_HUMAN
Immunogen Region: 19-575
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-575 of human CD141/Thrombomodulin (NP_000352.1).
Immunogen Sequence: APAEPQPGGSQCVEHDCFAL YPGPATFLNASQICDGLRGH LMTVRSSVAADVISLLLNGD GGVGRRRLWIGLQLPPGCGD PKRLGPLRGFQWVTGDNNTS YSRWARLDLNGAPLCGPLCV AVSAAEATVPSEPIWEEQQC EVKADGFLCEFHFPATCRPL AVEPGAAAAAVSITYGTPFA ARGADFQALPVGSSAAVAPL GLQLMCTAPPGAVQGHWARE APGAWDCSVENGGCEHACN
Tissue Specificity Endothelial cells are unique in synthesizing thrombomodulin.
Post Translational Modifications N-glycosylated. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Protein Name Thrombomodulin
Tm
Fetomodulin
Cd Antigen Cd141
Database Links Reactome: R-HSA-140875
Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Thrombomodulin antibody
Anti-Tm antibody
Anti-Fetomodulin antibody
Anti-Cd Antigen Cd141 antibody
Anti-THBD antibody
Anti-THRM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance