Anti-TFE3 antibody [ZR365] (STJ180543)

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STJ180543

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Host: Rabbit
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TFE3 is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZR365
Conjugation: Unconjugated
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100-200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: TFE3
Gene ID: 7030
Uniprot ID: TFE3_HUMAN
Tissue Specificity Ubiquitous in fetal and adult tissues.
Post Translational Modifications Sumoylated.does not affect dimerization with MITF. Phosphorylation ar Ser-47 and Ser-321 by MTOR via non-canonical mTORC1 pathway regulates its stability and subcellular location, respectively. When nutrients are present, phosphorylation by MTOR at Ser-47 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. When nutrients are present, phosphorylation by MTOR at Ser-321 also promotes association with 14-3-3/YWHA adapters and retention in the cytosol. Phosphorylation at Ser-47 plays a more critical role than phosphorylation at Ser-321 for TFE3 inactivation. Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and transcription factor activity. Ubiquitinated by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-47 by MTOR, leading to its degradation by the proteasome.
Function Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3').efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB.mTOR-dependent TFE3 cytosolic retention and inactivation promotes exit from pluripotency. Required to maintain the naive pluripotent state of hematopoietic stem cell.mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency. TFE3 activity is also involved in the inhibition of neuronal progenitor differentiation. Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes.mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. Promotes TGF-beta-induced transcription of COL1A2.via its interaction with TSC22D1 at E-boxes in the gene proximal promoter. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.
Protein Name Transcription Factor E3
Class E Basic Helix-Loop-Helix Protein 33
Bhlhe33
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Lysosome Membrane
When Nutrients Are Present
Recruited To The Lysosomal Membrane Via Association With Gdp-Bound Ragc/Rragc (Or Ragd/Rragd): It Is Then Phosphorylated By Mtor
Phosphorylation By Mtor Prevents Nuclear Translocation And Promotes Ubiquitination And Degradation
Conversely
Inhibition Of Mtorc1
Starvation And Lysosomal Disruption
Promotes Dephosphorylation And Translocation To The Nucleus
Alternative Antibody Names Anti-Transcription Factor E3 antibody
Anti-Class E Basic Helix-Loop-Helix Protein 33 antibody
Anti-Bhlhe33 antibody
Anti-TFE3 antibody
Anti-BHLHE33 antibody

Information sourced from Uniprot.org

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