| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-TFE3 (191-440) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S0MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TFE3 |
| Gene ID: |
7030 |
| Uniprot ID: |
TFE3_HUMAN |
| Immunogen Region: |
191-440 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 191-440 of human TFE3 (P19532). |
| Immunogen Sequence: |
LQQARRQQVKQYLSTTLGPK LASQALTPPPGPASAQPLPA PEAAHTTGPTGSAPNSPMAL LTIGSSSEKEIDDVIDEIIS LESSYNDEMLSYLPGGTTGL QLPSTLPVSGNLLDVYSSQG VATPAITVSNSCPAELPNIK REISETEAKALLKERQKKDN HNLIERRRRFNINDRIKELG TLIPKSSDPEMRWNKGTILK ASVDYIRKLQKEQQRSKDLE SRQRSLEQANRSLQLRIQE |
| Tissue Specificity | Ubiquitous in fetal and adult tissues. |
| Post Translational Modifications | Sumoylated.does not affect dimerization with MITF. Phosphorylation ar Ser-47 and Ser-321 by MTOR via non-canonical mTORC1 pathway regulates its stability and subcellular location, respectively. When nutrients are present, phosphorylation by MTOR at Ser-47 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. When nutrients are present, phosphorylation by MTOR at Ser-321 also promotes association with 14-3-3/YWHA adapters and retention in the cytosol. Phosphorylation at Ser-47 plays a more critical role than phosphorylation at Ser-321 for TFE3 inactivation. Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and transcription factor activity. Ubiquitinated by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-47 by MTOR, leading to its degradation by the proteasome. |
| Function | Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3').efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB.mTOR-dependent TFE3 cytosolic retention and inactivation promotes exit from pluripotency. Required to maintain the naive pluripotent state of hematopoietic stem cell.mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency. TFE3 activity is also involved in the inhibition of neuronal progenitor differentiation. Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes.mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. Promotes TGF-beta-induced transcription of COL1A2.via its interaction with TSC22D1 at E-boxes in the gene proximal promoter. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. |
| Protein Name | Transcription Factor E3Class E Basic Helix-Loop-Helix Protein 33Bhlhe33 |
| Cellular Localisation | CytoplasmCytosolNucleusLysosome MembraneWhen Nutrients Are PresentRecruited To The Lysosomal Membrane Via Association With Gdp-Bound Ragc/Rragc (Or Ragd/Rragd): It Is Then Phosphorylated By MtorPhosphorylation By Mtor Prevents Nuclear Translocation And Promotes Ubiquitination And DegradationConverselyInhibition Of Mtorc1Starvation And Lysosomal DisruptionPromotes Dephosphorylation And Translocation To The Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E3 antibodyAnti-Class E Basic Helix-Loop-Helix Protein 33 antibodyAnti-Bhlhe33 antibodyAnti-TFE3 antibodyAnti-BHLHE33 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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