• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500
  • Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, mtTFA Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TFAM antibody (131-180) (STJ94281)

SKU:
STJ94281

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse/Bovine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription factor A, mitochondrial (131-180) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFAM
Gene ID: 7019
Uniprot ID: TFAM_HUMAN
Immunogen Region: 131-180
Specificity: mtTFA Polyclonal Antibody detects endogenous levels of mtTFA protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human TFAM. AA range:131-180
Post Translational Modifications Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.
Function Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Protein Name Transcription Factor A - Mitochondrial
Mttfa
Mitochondrial Transcription Factor 1
Mttf1
Transcription Factor 6
Tcf-6
Transcription Factor 6-Like 2
Database Links Reactome: R-HSA-163282
Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Transcription Factor A - Mitochondrial antibody
Anti-Mttfa antibody
Anti-Mitochondrial Transcription Factor 1 antibody
Anti-Mttf1 antibody
Anti-Transcription Factor 6 antibody
Anti-Tcf-6 antibody
Anti-Transcription Factor 6-Like 2 antibody
Anti-TFAM antibody
Anti-TCF6 antibody
Anti-TCF6L2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance