| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TFAM (1-220) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TFAM |
| Gene ID: |
7019 |
| Uniprot ID: |
TFAM_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1). |
| Immunogen Sequence: |
MAFLRSMWGVLSALGRSGAE LCTGCGSRLRSPFSFVYLPR WFSSVLASCPKKPVSSYLRF SKEQLPIFKAQNPDAKTTEL IRRIAQRWRELPDSKKKIYQ DAYRAEWQVYKEEISRFKEQ LTPSQIMSLEKEIMDKHLKR KAMTKKKELTLLGKPKRPRS AYNVYVAERFQEAKGDSPQE KLKTVKENWKNLSDSEKELY IQHAKEDETRYHNEMKSWEE |
| Post Translational Modifications | Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease. |
| Function | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. |
| Protein Name | Transcription Factor A - MitochondrialMttfaMitochondrial Transcription Factor 1Mttf1Transcription Factor 6Tcf-6Transcription Factor 6-Like 2 |
| Database Links | Reactome: R-HSA-163282Reactome: R-HSA-2151201 |
| Cellular Localisation | MitochondrionMitochondrion MatrixMitochondrion Nucleoid |
| Alternative Antibody Names | Anti-Transcription Factor A - Mitochondrial antibodyAnti-Mttfa antibodyAnti-Mitochondrial Transcription Factor 1 antibodyAnti-Mttf1 antibodyAnti-Transcription Factor 6 antibodyAnti-Tcf-6 antibodyAnti-Transcription Factor 6-Like 2 antibodyAnti-TFAM antibodyAnti-TCF6 antibodyAnti-TCF6L2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
