• Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-tonsilla, antibody was diluted at 1:100
  • Western blot analysis of HeLa, HepG2 cells using TERT Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-TERT antibody (931-980 aa) (STJ96879)

SKU:
STJ96879

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Telomerase reverse transcriptase (931-980 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TERT
Gene ID: 7015
Uniprot ID: TERT_HUMAN
Immunogen Region: 931-980 aa
Specificity: TERT Polyclonal Antibody detects endogenous levels of TERT protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human TERT at the amino acid range 931-980
Post Translational Modifications Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation at Ser-227 by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-457 by DYRK2 promotes ubiquitination by the EDVP complex and degradation. Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-457 by DYRK2. Ubiquitinated leads to proteasomal degradation. (Microbial infection) In case of infection by HIV-1, the EDVP complex is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP, leading to ubiquitination and degradation.
Function Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Protein Name Telomerase Reverse Transcriptase
Hest2
Telomerase Catalytic Subunit
Telomerase-Associated Protein 2
Tp2
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-201722
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Chromosome
Telomere
Cytoplasm
Pml Body
Shuttling Between Nuclear And Cytoplasm Depends On Cell Cycle
Phosphorylation States
Transformation And Dna Damage
Diffuse Localization In The Nucleoplasm
Enriched In Nucleoli Of Certain Cell Types
Translocated To The Cytoplasm Via Nuclear Pores In A Crm1/Ran-Dependent Manner Involving Oxidative Stress-Mediated Phosphorylation At Tyr-707
Dephosphorylation At This Site By Shp2 Retains Tert In The Nucleus
Translocated To The Nucleus By Phosphorylation By Akt
Alternative Antibody Names Anti-Telomerase Reverse Transcriptase antibody
Anti-Hest2 antibody
Anti-Telomerase Catalytic Subunit antibody
Anti-Telomerase-Associated Protein 2 antibody
Anti-Tp2 antibody
Anti-TERT antibody
Anti-EST2 antibody
Anti-TCS1 antibody
Anti-TRT antibody

Information sourced from Uniprot.org

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