| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Tyrosyl-Dna Phosphodiesterase 2 (1-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TDP2 |
| Gene ID: |
51567 |
| Uniprot ID: |
TYDP2_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TDP2 (NP_057698.2). |
| Immunogen Sequence: |
MELGSCLEGGREAAEEEGEP EVKKRRLLCVEFASVASCDA AVAQCFLAENDWEMERALNS YFEPPVEESALERRPETISE PKTYVDLTNEETTDSTTSKI SPSEDTQQENGSMFSLITWN IDGLDLNNLSERARGVCSYL ALYSPDVIFLQEVIPPYYSY LKKRSSNYEIITGHEEGYFT AIMLKKSRVKLKSQEIIPFP |
| Tissue Specificity | Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum. |
| Post Translational Modifications | Ubiquitinated by TRAF6. |
| Function | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. |
| Protein Name | Tyrosyl-Dna Phosphodiesterase 2Tyr-Dna Phosphodiesterase 2Htdp25'-Tyrosyl-Dna Phosphodiesterase5'-Tyr-Dna PhosphodiesteraseEts1-Associated Protein 2Ets1-Associated Protein IiEapiiTraf And Tnf Receptor-Associated ProteinTyrosyl-Rna PhosphodiesteraseVpg Unlinkase |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusPml BodyNucleolusCytoplasmLocalizes To Nucleolar Cavities Following StressLocalization To Nucleolus Is Dependent On Pml Protein(Microbial Infection) In Case Of Infection By PicornavirusRelocalizes To Cytoplasmic Sites Distinct From Those Containing Viral Proteins Associated With Rna Replication Or Encapsidation |
| Alternative Antibody Names | Anti-Tyrosyl-Dna Phosphodiesterase 2 antibodyAnti-Tyr-Dna Phosphodiesterase 2 antibodyAnti-Htdp2 antibodyAnti-5'-Tyrosyl-Dna Phosphodiesterase antibodyAnti-5'-Tyr-Dna Phosphodiesterase antibodyAnti-Ets1-Associated Protein 2 antibodyAnti-Ets1-Associated Protein Ii antibodyAnti-Eapii antibodyAnti-Traf And Tnf Receptor-Associated Protein antibodyAnti-Tyrosyl-Rna Phosphodiesterase antibodyAnti-Vpg Unlinkase antibodyAnti-TDP2 antibodyAnti-EAP2 antibodyAnti-TTRAP antibodyAnti-AD-022 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TDP2 knockout (KO) HeLa cells, using [KO Validated] TDP2 Rabbit polyclonal antibody (STJ119990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98775/382823/STJ119990_1__79288.1713151323.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TDP2 knockout (KO) HeLa cells, using [KO Validated] TDP2 Rabbit polyclonal antibody (STJ119990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
