• STJ70509 (1µg/ml) staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70509 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70509 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70509 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-TCP1 antibody (C-Term) (STJ70509)

SKU:
STJ70509-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-TCP1 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-1-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TCP1
Gene ID: 6950
Uniprot ID: TCPA_HUMAN
Immunogen Region: C-Term
Accession Number: NP_110379.2; NP_001008897.1
Specificity: This antibody is expected to recognise both reported isoforms.
Immunogen Sequence: SYEDAVHSGALND
Protein Name T-Complex Protein 1 Subunit Alpha
Tcp-1-Alpha
Cct-Alpha
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6814122
Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Alpha antibody
Anti-Tcp-1-Alpha antibody
Anti-Cct-Alpha antibody
Anti-TCP1 antibody
Anti-CCT1 antibody
Anti-CCTA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance