• Western blot analysis of Jurkat, using E2A/TCF3 Rabbit monoclonal antibody (STJ11105262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of Mouse ovary, using E2A/TCF3 Rabbit monoclonal antibody (STJ11105262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TCF3 antibody (379-525) [S5262RM] (STJ11105262)

SKU:
STJ11105262

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Transcription Factor E2-Alpha (379-525) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5262RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCF3
Gene ID: 6929
Uniprot ID: TFE2_HUMAN
Immunogen Region: 379-525
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 379-525 of human E2A/TCF3 (NP_003191.1).
Immunogen Sequence: SPSYDGGLHGLQSKIEDHLD EAIHVLRSHAVGTAGDMHTL LPGHGALASGFTGPMSLGGR HAGLVGGSHPEDGLAGSTSL MHNHAALPSQPGTLPDLSRP PDSYSGLGRAGATAAASEIK REEKEDEENTSAADHSEEEK KELKAPR
Post Translational Modifications Phosphorylated following NGF stimulation. Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes.
Function Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. Isoform E47: Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
Protein Name Transcription Factor E2-Alpha
Class B Basic Helix-Loop-Helix Protein 21
Bhlhb21
Immunoglobulin Enhancer-Binding Factor E12/E47
Immunoglobulin Transcription Factor 1
Kappa-E2-Binding Factor
Transcription Factor 3
Tcf-3
Transcription Factor Itf-1
Database Links Reactome: R-HSA-525793
Reactome: R-HSA-8939236
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2-Alpha antibody
Anti-Class B Basic Helix-Loop-Helix Protein 21 antibody
Anti-Bhlhb21 antibody
Anti-Immunoglobulin Enhancer-Binding Factor E12/E47 antibody
Anti-Immunoglobulin Transcription Factor 1 antibody
Anti-Kappa-E2-Binding Factor antibody
Anti-Transcription Factor 3 antibody
Anti-Tcf-3 antibody
Anti-Transcription Factor Itf-1 antibody
Anti-TCF3 antibody
Anti-BHLHB21 antibody
Anti-E2A antibody
Anti-ITF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance