STJ71080 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TCF1/HNF1 antibody (Internal) (STJ71080)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71080-100
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General Information

Short DescriptionGoat polyclonal antibody anti-TCF1/HNF1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:4000.
WB: A customer reported a weak band at approx. 75kDa on lysate of cell line Caco-2, at a concentration of 3ug/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolHNF1A
Gene ID6927
Uniprot IDHNF1A_HUMAN
Accession NumberNP_000536.3
Immunogen RegionInternal
Immunogen SequenceDELPTKKGRRNRFK

Additional Info

Post Translational Modifications Ubiquitinated in s SPOP-dependent manner.leading to prteasomal degradation.
Function Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with NR5A2 to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
Protein Name Hepatocyte Nuclear Factor 1-Alpha
Hnf-1-Alpha
Hnf-1a
Liver-Specific Transcription Factor Lf-B1
Lfb1
Transcription Factor 1
Tcf-1
Database Links Reactome: R-HSA-210745
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 1-Alpha antibody
Anti-Hnf-1-Alpha antibody
Anti-Hnf-1a antibody
Anti-Liver-Specific Transcription Factor Lf-B1 antibody
Anti-Lfb1 antibody
Anti-Transcription Factor 1 antibody
Anti-Tcf-1 antibody
Anti-HNF1A antibody
Anti-TCF1 antibody

Information sourced from Uniprot.org

Citations

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