• Western blot analysis of extracts of HeLa cells, using TBC1D1 antibody (STJ113650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using TBC1D1 antibody (STJ113650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using TBC1D1 antibody (STJ113650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of HeLa cells, using TBC1D1 antibody (STJ113650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TBC1D1 antibody (1069-1168) (STJ113650)

SKU:
STJ113650

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TBC1D1 (1069-1168) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBC1D1
Gene ID: 23216
Uniprot ID: TBCD1_HUMAN
Immunogen Region: 1069-1168
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1069-1168 of human TBC1D1 (NP_055988.2).
Immunogen Sequence: IDSSPLSDNQRMDKLEKTNS SLRKQNLDLLEQLQVANGRI QSLEATIEKLLSSESKLKQA MLTLELERSALLQTVEELRR RSAEPSDREPECTQPEPTGD
Function May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.
Protein Name Tbc1 Domain Family Member 1
Database Links Reactome: R-HSA-1445148
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Tbc1 Domain Family Member 1 antibody
Anti-TBC1D1 antibody
Anti-KIAA1108 antibody

Information sourced from Uniprot.org

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