| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TAF12 (1-161) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TAF12 |
| Gene ID: |
6883 |
| Uniprot ID: |
TAF12_HUMAN |
| Immunogen Region: |
1-161 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1). |
| Immunogen Sequence: |
MNQFGPSALINLSNFSSIKP EPASTPPQGSMANSTAVVKI PGTPGAGGRLSPENNQVLTK KKLQDLVREVDPNEQLDEDV EEMLLQIADDFIESVVTAAC QLARHRKSSTLEVKDVQLHL ERQWNMWIPGFGSEEIRPYK KACTTEAHKQRMALIRKTTK K |
| Tissue Specificity | Ubiquitous. |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. |
| Protein Name | Transcription Initiation Factor Tfiid Subunit 12Transcription Initiation Factor Tfiid 20/15 Kda SubunitsTafii-20/Tafii-15Tafii20/Tafii15 |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-3214847Reactome: R-HSA-674695Reactome: R-HSA-6804756Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Initiation Factor Tfiid Subunit 12 antibodyAnti-Transcription Initiation Factor Tfiid 20/15 Kda Subunits antibodyAnti-Tafii-20/Tafii-15 antibodyAnti-Tafii20/Tafii15 antibodyAnti-TAF12 antibodyAnti-TAF15 antibodyAnti-TAF2J antibodyAnti-TAFII20 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TAF12 knockout (KO) HeLa cells, using [KO Validated] TAF12 Rabbit polyclonal antibody (STJ11100046) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89118/357570/STJ11100046_1__33488.1713121698.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TAF12 knockout (KO) HeLa cells, using [KO Validated] TAF12 Rabbit polyclonal antibody (STJ11100046) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
