• Immunoprecipitation analysis of 600 Mu g extracts of Mouse heart using 3 Mu g SUPT6H antibody (STJ118862). Western blot was performed from the immunoprecipitate using SUPT6H antibody (STJ118862) at a dilution of 1:500.
  • Immunofluorescence analysis of U-2 OS cells using SUPT6H Rabbit polyclonal antibody (STJ118862) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SUPT6H Rabbit polyclonal antibody (STJ118862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SUPT6H antibody (1500-1726) (STJ118862)

SKU:
STJ118862

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUPT6H (1500-1726) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPT6H
Gene ID: 6830
Uniprot ID: SPT6H_HUMAN
Immunogen Region: 1500-1726
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1500-1726 of human SUPT6H (NP_003161.2).
Immunogen Sequence: IFPTVNGLFRWFKDHYQDPV PGITPSSSSRTRTPASINAT PANINLADLTRAVNALPQNM TSQMFSAIAAVTGQGQNPNA TPAQWASSQYGYGGSGGGSS AYHVFPTPAQQPVATPLMTP SYSYTTPSQPITTPQYHQLQ ASTTPQSAQAQPQPSSSSRQ RQQQPKSNSHAAIDWGKMAE QWLQEKEAERRKQKQRLTPR PSPSPMIESTPMSIAGDATP LLDEMDR
Tissue Specificity Ubiquitously expressed.
Function Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.
Protein Name Transcription Elongation Factor Spt6
Hspt6
Histone Chaperone Suppressor Of Ty6
Tat-Cotransactivator 2 Protein
Tat-Ct2 Protein
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-674695
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Elongation Factor Spt6 antibody
Anti-Hspt6 antibody
Anti-Histone Chaperone Suppressor Of Ty6 antibody
Anti-Tat-Cotransactivator 2 Protein antibody
Anti-Tat-Ct2 Protein antibody
Anti-SUPT6H antibody
Anti-KIAA0162 antibody
Anti-SPT6H antibody

Information sourced from Uniprot.org

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