• Immunohistochemistry analysis of paraffin-embedded mouse liver using SUPT20H antibody (STJ29511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using SUPT20H antibody (STJ29511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using SUPT20H antibody (STJ29511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SUPT20H antibody (STJ29511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SUPT20H antibody (580-779) (STJ29511)

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STJ29511

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUPT20H (580-779) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPT20H
Gene ID: 55578
Uniprot ID: SP20H_HUMAN
Immunogen Region: 580-779
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2).
Immunogen Sequence: NLSGLLPSGGLLPNALPSAM QAASQAGVPFGLKNTSSLRP LNLLQLPGGSLIFNTLQQQQ QQLSQFTPQQPQQPTTCSPQ QPGEQGSEQGSTSQEQALSA QQAAVINLTGVGSFMQSQAA VLSQLGSAENRPEQSLPQQR FQLSSAFQQQQQQIQQLRFL QHQMAMAAAAAQTAQLHHHR HTGSQSKSKMKRGTPTTPKF
Tissue Specificity Highly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues.
Function Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation-induced ATG9A trafficking during autophagy.
Protein Name Transcription Factor Spt20 Homolog
P38-Interacting Protein
P38ip
Database Links Reactome: R-HSA-3214847
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Spt20 Homolog antibody
Anti-P38-Interacting Protein antibody
Anti-P38ip antibody
Anti-SUPT20H antibody
Anti-C13orf19 antibody
Anti-FAM48A antibody
Anti-FP757 antibody

Information sourced from Uniprot.org

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