• Western blot analysis of extracts of various cell lines, using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using SUMO2/3 rabbit monoclonal antibody (STJ11102236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SUMO2 antibody (1-95) [S6MR] (STJ11102236)

SKU:
STJ11102236

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:200-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMO3
SUMO2
Gene ID: 6612
6613
Uniprot ID: SUMO3_HUMAN
SUMO2_HUMAN
Immunogen Region: 1-95
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-95 of human SUMO2/3 (P61956).
Immunogen Sequence: MADEKPKEGVKTENNDHINL KVAGQDGSVVQFKIKRHTPL SKLMKAYCERQGLSMRQIRF RFDGQPINETDTPAQLEMED EDTIDVFQQQTGGVY
Tissue Specificity P55854 - Expressed predominantly in liver.
P61956 - Broadly expressed.
Alternative Antibody Names Anti-Small Ubiquitin-Related Modifier 3 antibody
Anti-Sumo-3 antibody
Anti-Smt3 Homolog 1 antibody
Anti-Sumo-2 antibody
Anti-Ubiquitin-Like Protein Smt3a antibody
Anti-Smt3a antibody
Anti-SUMO3 antibody
Anti-SMT3A antibody
Anti-SMT3H1 antibody
Anti-Small Ubiquitin-Related Modifier 2 antibody
Anti-Sumo-2 antibody
Anti-Hsmt3 antibody
Anti-Smt3 Homolog 2 antibody
Anti-Sumo-3 antibody
Anti-Sentrin-2 antibody
Anti-Ubiquitin-Like Protein Smt3b antibody
Anti-Smt3b antibody
Anti-SUMO2 antibody
Anti-SMT3B antibody
Anti-SMT3H2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance