| Short Description : | Rabbit monoclonal anti-SUMO Conjugating Enzyme UBC9 for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | R01-6I6 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.3 mg/mL |
| Purification: | Affinity Purified |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | UBE2I |
| Gene ID: | 7329 |
| Uniprot ID: | UBC9_HUMAN |
| Immunogen: | A synthetic peptide of human UBE2I/UBC9 |
| Tissue Specificity | Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
| Post Translational Modifications | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. |
| Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. |
| Protein Name | Sumo-Conjugating Enzyme Ubc9Ring-Type E3 Sumo Transferase Ubc9Sumo-Protein LigaseUbiquitin Carrier Protein 9Ubiquitin Carrier Protein IUbiquitin-Conjugating Enzyme E2 IUbiquitin-Protein Ligase IP18 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-196791Reactome: R-HSA-3065678Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-4655427Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-8866904Reactome: R-HSA-9615933Reactome: R-HSA-9683610Reactome: R-HSA-9694631Reactome: R-HSA-9735871Reactome: R-HSA-9793242Reactome: R-HSA-9833482Reactome: R-HSA-9843940Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly NuclearIn SpermatocytesLocalizes In Synaptonemal ComplexesRecruited By Bcl11a Into The Nuclear Body |
| Alternative Antibody Names | Anti-Sumo-Conjugating Enzyme Ubc9 antibodyAnti-Ring-Type E3 Sumo Transferase Ubc9 antibodyAnti-Sumo-Protein Ligase antibodyAnti-Ubiquitin Carrier Protein 9 antibodyAnti-Ubiquitin Carrier Protein I antibodyAnti-Ubiquitin-Conjugating Enzyme E2 I antibodyAnti-Ubiquitin-Protein Ligase I antibodyAnti-P18 antibodyAnti-UBE2I antibodyAnti-UBC9 antibodyAnti-UBCE9 antibody |
Information sourced from Uniprot.org
