| Host: | Mouse |
| Applications: | ELISA/WB/ICC/IF/IHC/FACS |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-SUMO 2/3 (1-93aa) for use in ELISA, WB, ICC, IF, IHC and FACS in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | AT10F1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2bk |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Purification: | By protein-G affinity chromatography |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SUMO2 |
| Gene ID: | 6613 |
| Uniprot ID: | SUMO2_HUMAN |
| Immunogen Region: | 1-93aa |
| Accession Number: | NP_008868 |
| Immunogen: | Recombinant human SUMO2 (1-93aa) purified from E.coli |
| Tissue Specificity | Broadly expressed. |
| Post Translational Modifications | Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4. Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer. |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX. |
| Protein Name | Small Ubiquitin-Related Modifier 2Sumo-2Hsmt3Smt3 Homolog 2Sumo-3Sentrin-2Ubiquitin-Like Protein Smt3bSmt3b |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-3065676Reactome: R-HSA-3065678Reactome: R-HSA-3065679Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-9843940 |
| Cellular Localisation | NucleusPml Body |
| Alternative Antibody Names | Anti-Small Ubiquitin-Related Modifier 2 antibodyAnti-Sumo-2 antibodyAnti-Hsmt3 antibodyAnti-Smt3 Homolog 2 antibodyAnti-Sumo-3 antibodyAnti-Sentrin-2 antibodyAnti-Ubiquitin-Like Protein Smt3b antibodyAnti-Smt3b antibodyAnti-SUMO2 antibodyAnti-SMT3B antibodyAnti-SMT3H2 antibody |
Information sourced from Uniprot.org