• Whole extracts were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Strep-Tag Ⅱ (7A10) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Rosetta transfected with an empty expression vector Lane 2: Rosetta transfected with a Strep-TagⅡ-tagged MBP proteinPredicted band size: 55kDaObserved band size: 55kDa
  • Strep-Tag Ⅱ was immunoprecipitated from Strep-Tag Ⅱ-MBP (Rosetta) lysate with anti-Strep-Tag Ⅱ antibody. Western blot was performed on the immunoprecipitate using anti-Strep-Tag Ⅱ antibody, and followed by the HRP-conjugated Goat anti- mouse IgG Light chain antibody. Lane 1: Strep-Tag Ⅱ-MBP (Rosetta) lysate Lane 2: anti-Strep-Tag Ⅱ antibody IP in Strep-Tag Ⅱ-MBP (Rosetta) lysate Lane 3: mouse monoclonal IgG (MNH209) in Strep-Tag Ⅱ-MBP (Rosetta) lysate.
  • 1ug Strep II fusion protein+ primary antibody dilution at 1) 1:50002) 1:10000

Anti-Strep-Tag antibody [7A10] (STJ96918)

SKU:
STJ96918

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Strep-Tag-Tag is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 7A10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: The antibody detects Strep tag fusion proteins.
Immunogen: Synthetic Peptide of Strep-Tag
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance