• Western blot analysis of extracts of various cell lines, using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human testis using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat colon using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using Stathmin 1 rabbit monoclonal antibody (STJ11102063) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of frozen sections of mouse brain using Stathmin 1 rabbit monoclonal antibody (STJ11102063, dilution 1:100) followed by incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-STMN1 antibody (70-149) [S3MR] (STJ11102063)

SKU:
STJ11102063

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Stathmin 1 (70-149) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STMN1
Gene ID: 3925
Uniprot ID: STMN1_HUMAN
Immunogen Region: 70-149
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-149 of human Stathmin 1 (P16949).
Immunogen Sequence: KQLAEKREHEKEVLQKAIEE NNNFSKMAEEKLTHKMEANK ENREAQMAAKLERLREKDKH IEEVRKNKESKDPADETEAD
Tissue Specificity Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Post Translational Modifications Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Function Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Protein Name Stathmin
Leukemia-Associated Phosphoprotein P18
Metablastin
Oncoprotein 18
Op18
Phosphoprotein P19
Pp19
Prosolin
Protein Pr22
Pp17
Database Links Reactome: R-HSA-1251985
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Stathmin antibody
Anti-Leukemia-Associated Phosphoprotein P18 antibody
Anti-Metablastin antibody
Anti-Oncoprotein 18 antibody
Anti-Op18 antibody
Anti-Phosphoprotein P19 antibody
Anti-Pp19 antibody
Anti-Prosolin antibody
Anti-Protein Pr22 antibody
Anti-Pp17 antibody
Anti-STMN1 antibody
Anti-C1orf215 antibody
Anti-LAP18 antibody
Anti-OP18 antibody

Information sourced from Uniprot.org

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