• Western blot analysis of extracts of various cell lines, using STAG1 antibody (STJ119445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-STAG1 antibody (930-1140) (STJ119445)

SKU:
STJ119445

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STAG1 (930-1140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAG1
Gene ID: 10274
Uniprot ID: STAG1_HUMAN
Immunogen Region: 930-1140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 930-1140 of human STAG1 (NP_005853.2).
Immunogen Sequence: LFNELVQEQGPNLDRTSAHV SGIKELARRFALTFGLDQIK TREAVATLHKDGIEFAFKYQ NQKGQEYPPPNLAFLEVLSE FSSKLLRQDKKTVHSYLEKF LTEQMMERREDVWLPLISYR NSLVTGGEDDRMSVNSGSSS SKTSSVRNKKGRPPLHKKRV EDESLDNTWLNRTDTMIQTP GPLPAPQLTSTVLRENSRPM GDQIQEPESEH
Post Translational Modifications Phosphorylated by PLK1. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
Function Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Protein Name Cohesin Subunit Sa-1
Scc3 Homolog 1
Stromal Antigen 1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Chromosome
Centromere
Associates With Chromatin
Before Prophase It Is Scattered Along Chromosome Arms
During Prophase
Most Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By Plk1
Except At Centromeres
Where Cohesin Complexes Remain
At Anaphase
The Rad21 Subunit Of Cohesin Is Cleaved
Leading To The Dissociation Of The Complex From Chromosomes
Allowing Chromosome Separation
Alternative Antibody Names Anti-Cohesin Subunit Sa-1 antibody
Anti-Scc3 Homolog 1 antibody
Anti-Stromal Antigen 1 antibody
Anti-STAG1 antibody
Anti-SA1 antibody
Anti-SCC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance