Anti-SRSF3 antibody (1-90) (STJ27861)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SRSF3 (1-90) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SRSF3 |
| Gene ID | 6428 |
| Uniprot ID | SRSF3_HUMAN |
| Immunogen Region | 1-90 |
| Immunogen Sequence | MHRDSCPLDCKVYVGNLGNN GNKTELERAFGYYGPLRSVW VARNPPGFAFVEFEDPRDAA DAVRELDGRTLCGCRVRVEL SNGEKRSRNR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SRSF3 (NP_003008.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain. |
| Function | Splicing factor, which binds the consensus motif 5'-CACUAUCACUACC-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway).enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. |
| Protein Name | Serine/Arginine-Rich Splicing Factor 3Pre-Mrna-Splicing Factor Srp20Splicing Factor - Arginine/Serine-Rich 3 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmRecruited To Nuclear Speckles Following Interaction With Ythdc1 |
| Alternative Antibody Names | Anti-Serine/Arginine-Rich Splicing Factor 3 antibodyAnti-Pre-Mrna-Splicing Factor Srp20 antibodyAnti-Splicing Factor - Arginine/Serine-Rich 3 antibodyAnti-SRSF3 antibodyAnti-SFRS3 antibodyAnti-SRP20 antibody |
Information sourced from Uniprot.org