| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SRPK2 (420-520) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SRPK2 |
| Gene ID: |
6733 |
| Uniprot ID: |
SRPK2_HUMAN |
| Immunogen Region: |
420-520 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 420-520 of human SRPK2 (NP_872633.1). |
| Immunogen Sequence: |
NAESDYTYSSSYEQFNGELP NGRHKIPESQFPEFSTSLFS GSLEPVACGSVLSEGSPLTE QEESSPSHDRSRTVSASSTG DLPKAKTRAADLLVNPLDPR N |
| Tissue Specificity | Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney. |
| Post Translational Modifications | Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage. Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis. |
| Function | Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. |
| Protein Name | Srsf Protein Kinase 2Sfrs Protein Kinase 2Serine/Arginine-Rich Protein-Specific Kinase 2Sr-Protein-Specific Kinase 2 Cleaved Into - Srsf Protein Kinase 2 N-Terminal - Srsf Protein Kinase 2 C-Terminal |
| Database Links | Reactome: R-HSA-9694631 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleus SpeckleChromosomeShuttles Between The Nucleus And The CytoplasmKat5/Tip60 Inhibits Its Nuclear TranslocationPhosphorylation At Thr-492 By Pkb/Akt1 Promotes Nuclear TranslocationPreferentially Localizes Across The Entire Gene Coding RegionDuring TranscriptionAccumulates At Chromatin Loci Where Unscheduled R-Loops Form And Colocalizes With Paused 'Ser-5'-Phosphorlyated Polr2a/Rna Polymerase Ii And Helicase Ddx23 |
| Alternative Antibody Names | Anti-Srsf Protein Kinase 2 antibodyAnti-Sfrs Protein Kinase 2 antibodyAnti-Serine/Arginine-Rich Protein-Specific Kinase 2 antibodyAnti-Sr-Protein-Specific Kinase 2 Cleaved Into - Srsf Protein Kinase 2 N-Terminal - Srsf Protein Kinase 2 C-Terminal antibodyAnti-SRPK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
