• Immunofluorescence analysis of paraffin-embedded mouse lung using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat bone marrow using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of alpha 1 Spectrin in paraffin-embedded mouse liver using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of alpha 1 Spectrin in paraffin-embedded rat spleen using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of alpha 1 Spectrin in paraffin-embedded human tonsil using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPTA1 antibody (940-1160) (STJ114233)

SKU:
STJ114233

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPTA1 (940-1160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPTA1
Gene ID: 6708
Uniprot ID: SPTA1_HUMAN
Immunogen Region: 940-1160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human alpha 1 Spectrin (NP_003117.2).
Immunogen Sequence: KHEAFLLDLNSFGDSMKALR NQANACQQQQAAPVEGVAGE QRVMALYDFQARSPREVTMK KGDVLTLLSSINKDWWKVEA ADHQGIVPAVYVRRLAHDEF PMLPQRRREEPGNITQRQEQ IENQYRSLLDRAEERRRRLL QRYNEFLLAYEAGDMLEWIQ EKKAENTGVELDDVWELQKK FDEFQKDLNTNEPRLRDINK VADDLLFEGLLTPEGAQIRQ E
Function Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Protein Name Spectrin Alpha Chain - Erythrocytic 1
Erythroid Alpha-Spectrin
Database Links Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6807878
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Alternative Antibody Names Anti-Spectrin Alpha Chain - Erythrocytic 1 antibody
Anti-Erythroid Alpha-Spectrin antibody
Anti-SPTA1 antibody
Anti-SPTA antibody

Information sourced from Uniprot.org

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