| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
SPRED2 |
| Gene ID: |
200734 |
| Uniprot ID: |
SPRE2_HUMAN |
| Immunogen Region: |
120-300 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-300 of human SPRED2 (NP_861449.2). |
| Immunogen Sequence: |
EDLIEGSTTSSSTIHNEAEL GDDDVFTTATDSSSNSSQKR EQPTRTISSPTSCEHRRIYT LGHLHDSYPTDHYHLDQPMP RPYRQVSFPDDDEEIVRINP REKIWMTGYEDYRHAPVRGK YPDPSEDADSSYVRFAKGEV PKHDYNYPYVDSSDFGLGED PKGRGGSVIKTQPSRGKSRR R |
| Tissue Specificity | Expressed in liver, skin, small intestine, salivary gland and prostate. |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ubiquitinated.leading to degradation by the proteasome. |
| Function | Negatively regulates Ras signaling pathways and downstream activation of MAP kinases. Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells. |
| Protein Name | Sprouty-Related - Evh1 Domain-Containing Protein 2Spred-2 |
| Database Links | Reactome: R-HSA-5658442Reactome: R-HSA-5658623Reactome: R-HSA-6802953 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic VesicleSecretory Vesicle MembraneCytoplasmDetected In The Cytoplasm Of The Stratum Spinosum CellsWhere It Is Associated With Cytoplasmic Vesicles That Are Supposed To Be Secretory Granules |
| Alternative Antibody Names | Anti-Sprouty-Related - Evh1 Domain-Containing Protein 2 antibodyAnti-Spred-2 antibodyAnti-SPRED2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
