Anti-SPP1 antibody (261-310 aa) (STJ94832)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Osteopontin at the amino acid range 261-310
General Information
| Short Description | Rabbit polyclonal anti-Osteopontin (261-310 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:100-500IF ICC 1:100-500ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPP1 |
| Gene ID | 6696 |
| Uniprot ID | OSTP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Osteopontin at the amino acid range 261-310 |
| Immunogen Region | 261-310 aa |
| Specificity | OPN Polyclonal Antibody detects endogenous levels of OPN protein. |
Additional Info
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275Reactome: R-HSA-9856532 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteopontin antibodyAnti-Bone Sialoprotein 1 antibodyAnti-Nephropontin antibodyAnti-Secreted Phosphoprotein 1 antibodyAnti-Spp-1 antibodyAnti-Urinary Stone Protein antibodyAnti-Uropontin antibodyAnti-SPP1 antibodyAnti-BNSP antibodyAnti-OPN antibodyAnti-PSEC0156 antibody |
Information sourced from Uniprot.org