| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Osteopontin (261-310 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:100-500 IF ICC 1:100-500 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPP1 |
| Gene ID: | 6696 |
| Uniprot ID: | OSTP_HUMAN |
| Immunogen Region: | 261-310 aa |
| Specificity: | OPN Polyclonal Antibody detects endogenous levels of OPN protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Osteopontin at the amino acid range 261-310 |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin Bone Sialoprotein 1 Nephropontin Secreted Phosphoprotein 1 Spp-1 Urinary Stone Protein Uropontin |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-186797 Reactome: R-HSA-216083 Reactome: R-HSA-381426 Reactome: R-HSA-8949275 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteopontin antibody Anti-Bone Sialoprotein 1 antibody Anti-Nephropontin antibody Anti-Secreted Phosphoprotein 1 antibody Anti-Spp-1 antibody Anti-Urinary Stone Protein antibody Anti-Uropontin antibody Anti-SPP1 antibody Anti-BNSP antibody Anti-OPN antibody Anti-PSEC0156 antibody |
Information sourced from Uniprot.org
