Host: |
Rabbit |
Applications: |
WB |
Reactivity: |
Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit monoclonal antibody anti-Osteopontin (200-300) is suitable for use in Western Blot research applications. |
Clonality: |
Monoclonal |
Clone ID: |
S9MR |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:500-1:2000 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
SPP1 |
Gene ID: |
6696 |
Uniprot ID: |
OSTP_HUMAN |
Immunogen Region: |
200-300 |
Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Osteopontin (P10451). |
Immunogen Sequence: |
GAYKAIPVAQDLNAPSDWDS RGKDSYETSQLDDQSAETHS HKQSRLYKRKANDESNEHSD VIDSQELSKVSREFHSHEFH SHEDMLVVDPKSKEEDKHLK F |
Tissue Specificity | Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma. |
Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275 |
Cellular Localisation | Secreted |
Alternative Antibody Names | Anti-Osteopontin antibodyAnti-Bone Sialoprotein 1 antibodyAnti-Nephropontin antibodyAnti-Secreted Phosphoprotein 1 antibodyAnti-Spp-1 antibodyAnti-Urinary Stone Protein antibodyAnti-Uropontin antibodyAnti-SPP1 antibodyAnti-BNSP antibodyAnti-OPN antibodyAnti-PSEC0156 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance