| Host: |
Rat |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-Sphingosine Kinase 1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M540 |
| Conjugation: |
Unconjugated |
| Isotype: |
N.T. |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:250IHCICC 1:200IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Sphk1 |
| Gene ID: |
20698 |
| Uniprot ID: |
SPHK1_MOUSE |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42, 50, and 60 kDa forms of SK1 in human HeLa cells. |
| Immunogen: |
Clone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2. |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Protein Name | Sphingosine Kinase 1Sk 1Spk 1Acetyltransferase Sphk1 |
| Database Links | Reactome: R-MMU-1660661Reactome: -MMU-390471Reactome: -MMU-5218921Reactome: -MMU-9009391 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinNucleusCell MembraneSynapseTranslocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent MannerBinds To Membranes Containing Negatively Charged Lipids But Not Neutral LipidsRecruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative Antibody Names | Anti-Sphingosine Kinase 1 antibodyAnti-Sk 1 antibodyAnti-Spk 1 antibodyAnti-Acetyltransferase Sphk1 antibodyAnti-Sphk1 antibodyAnti-Sk1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
