• Western blot analysis of extracts of various cell lines, using SPHK1 antibody (STJ11104702) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPHK1 antibody (1-160) [S4702RM] (STJ11104702)

SKU:
STJ11104702

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sphingosine Kinase 1 (1-160) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4702RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPHK1
Gene ID: 8877
Uniprot ID: SPHK1_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human SPHK1 (NP_001136074.1).
Immunogen Sequence: MDPAGGPRGVLPRPCRVLVL LNPRGGKGKALQLFRSHVQP LLAEAEISFTLMLTERRNHA RELVRSEELGRWDALVVMSG DGLMHEVVNGLMERPDWETA IQKPLCSLPAGSGNALAASL NHYAGYEQVTNEDLLTNCTL LLCRRLLSPMNLLSLHTASG
Tissue Specificity Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
Protein Name Sphingosine Kinase 1
Sk 1
Spk 1
Acetyltransferase Sphk1
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-390471
Reactome: R-HSA-5218921
Reactome: R-HSA-9009391
Cellular Localisation Cytoplasm
Nucleus
Cell Membrane
Endosome Membrane
Peripheral Membrane Protein
Membrane
Clathrin-Coated Pit
Synapse
Translocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent Manner
Binds To Membranes Containing Negatively Charged Lipids But Not Neutral Lipids
Recruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion
Alternative Antibody Names Anti-Sphingosine Kinase 1 antibody
Anti-Sk 1 antibody
Anti-Spk 1 antibody
Anti-Acetyltransferase Sphk1 antibody
Anti-SPHK1 antibody
Anti-SK1 antibody
Anti-SPHK antibody
Anti-SPK antibody

Information sourced from Uniprot.org

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